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Test Code LAB2477 UW ADV PCR Quant, BAL, Blood, CSF, or Non-Cellular Fluid

Important Note

This test requires a previous positive RpdUpperResp or RpdLowerResp PCR.

For BAL, Nasal wash or other respiratory specimens with QUALitative results, order RpdLowerResp or RpdUpperResp.

For bone marrow aspirate, tissue, and other specimen types on swabs with QUALitative results, order ADVQLT tissue/cell/swab.

Infectious

Additional Codes

ADVQN

Clinical System Name

UW ADV PCR Quant, BAL, Blood, CSF, or Non-Cellular Fluid

Synonyms

Adenoviral DNA; Adenovirus PCR; Adenoviruses by PCR Quantitative; PCR - Adenovirus; PCR-Adenoviruses

Sample Requirements

Specimen:

1 mL BAL, CSF, or Clear Non-Cellular Fluid

3 mL Whole Blood

 

Container(s):

Fluids: Sterile Screw-Capped Container, CSF Tube

Whole Blood:  Lavender/EDTA (preferred), Red Top

 

Preferred Vol: 1.0 mL Fluid, 3.0 mL Blood

Minimum Vol: 0.5 mL

Processing Instructions

Whole Blood: Separate plasma/serum from whole blood within 24 hours of collection.  Freeze plasma at -20 C.  Do not freeze/thaw.

 

BAL / CSF / Non-Cellular Fluid: Freeze at -20 C.  Do not freeze/thaw.

 

Reject due to: Presence of cellular material (ADVQLT should be ordered for cellular fluids), plasma collected in sodium or lithium heparin

Spin: N

Aliquot: N

Temp: -20 C

Storage Location: Freeze sample within TWO hours of collection. Avoid freeze/thaw cycles.
 

Note:

Monday - Friday: Place sample in the Send Outs rack in the CPA -20 freezer.

Saturday - Sunday and Holidays: Close batch in MayoACCESS and courier on dry ice to UW NW220.  (Call on Holidays to be sure the lab is open to accept samples).

 

Off-Site Collection: Freeze within TWO hours of collection. Avoid freeze/thaw cycles. If no freezer is available, ship at 4 C. Ship time is not to exceed 48 hours.

Stability

Specimen Type Temperature Time
BAL, Plasma/Serum, CSF, or Clear Non-Cellular Fluid Room Temp

Unacceptable
  Refrigerated < 48 h
  Frozen

Indefinite

 

Availability

STAT Performed TAT
N M, Th 5 - 7 d

 

Performing Laboratory

University of Washington Medical Center

Department of Laboratory Medicine

Eastlake Virology (EVIR)

1959 NE Pacific St, NW220
Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

87799 (x2)

Methodology

Method: Real-Time Polymerase Chain Reaction (PCR)

Analytical Volume: 0.5 mL BAL, Plasma/Serum, CSF, or Clear Non-Cellular Fluid

Reference Range

Reference values accompany patient report.

Send Out Instructions

Reference Test Name: Adenoviruses, PCR Quantitative
Reference Lab Test Code:

ADVQN
Instructions:

Send out daily with the UW courier.