Test Code LAB2506 Neisseria gonorrhoeae PCR
Synonyms
Gonorrhoeae PCR
Neisseria gonorrhoeae amplified probe
LAB2506
Sample Requirements
Specimen: Dirty Catch Urine*, Endocervical, Vaginal, Throat, Rectal
NOTE: For non-FDA approved specimens (Eye, Urethral, Penile), order a Miscellaneous Lab Test. Collect samples using the Apitma Unisex or Aptima Multitest Collection Kit. Eye samples will be sent to Mayo Clinic Laboratory for testing. Penile and Urethral samples will be sent to UWMC Laboratory for testing.
Container:
- Sterile Container for all Urines
- "GeneXpert Vaginal Endocervical Collection kit" CT/NG swabs for all in house PCR testing
- If sample is bloody collect instead:
- "Aptima Unisex Collection kit" for Endovervical or Urethral sites.
- "Aptima Multitest Collection kit" for Vaginal Penile, Throat, or Rectal Sites
Preferred Vol: 10mL for Urine
Minimum Vol: 7mL for Urine**
Please consult the Photo Swab Guide to determine which swab to collect.
*Dirty Catch urine is the first stream of the first morning void (for maximum sensitivity), or the first stream at least 1 hour after the last void. Patient should not perform any genital cleaning before collection. If the patient has submitted a clean catch sample, a dirty catch sample can be submitted at least one hour since the last void. Clean catch urines are unacceptable for testing and will be REJECTED.
**Urine samples with suboptimal volumes (between 2mL - 7mL) will be sent to the University of Washington for testing. Send urine to lab in sterile container. Urine samples of less than 2mL are unacceptable and will be REJECTED.
Bloody swabs/urine samples will be sent to University of Washington for testing. Send bloody urine to lab in sterile container. Collect bloody Vaginal or Endocervical samples with Gen-Probe APTIMA collection kit. Call Microbiology (206-987-2585) for APTIMA Kits.
Samples received in Aptima collection kits will be forwarded to the University of Washington for testing.
Processing Instructions
CPA: Send sample to Microbiology for processing.
Regional Clinic collection: Send to SCH Main Campus for testing. Refrigerate before and during courier transport. If urine sample received is less than minimal volume, call Microbiology at 206-987-2585 for consult on running or cancelling the test. Additional questions on collection and other specimen sites, contact CF/ Molecular Microbiology Supervisor or Director of Microbiology.
Stability
Urine | |
---|---|
Temp | Time |
Room temp | 24 hours |
Refrigerated | 1 week |
Frozen | N |
Xpert Vaginal/Endocervical Swabs | |
Temp | Time |
Room temp | 60 days |
Refrigerated | 60 days |
Frozen | N |
Aptima Multitest Swab Specimen Collection Kit | |
Temp | Time |
Room temp | 30 days |
Refrigerated | 30 days |
Frozen | N |
Aptima Unisex Swab Specimen Collection Kit | |
Temp | Time |
Room temp | 30 days |
Refrigerated | 30 days |
Frozen | N |
Availability
Procedure | Performed | Turn Around Time (TAT) |
---|---|---|
In-House PCR (Cepheid) | 24/7 | 3 hours |
APTIMA PCR (for bloody samples sent to UW for testing) | 24/7 |
3-5 days |
Performing Laboratory
Seattle Children's Laboratory
Department
Microbiology Laboratory
CPT Codes
87591
Description
This test includes nucleic acid amplification for Neisseria gonorrhoeae with amplified probe technique. It is the recommended testing platform for Neisseria gonorrhoeae because of the rapid turn around time and test sensitivity.
If an antibiotic sensitivity is needed, collect a BD E Swab for Genital Culture.
For forensic evaluation of Neisseria gonorrhoeae, please see Sexual Assault Photo Swab Guide and order CT GC PCR through the Sexual Assault PowerPlan.
Clincal System Name
Neisseria gonorrhoeae PCR
Reporting Information
Final Report (Cepheid): 3 hours
Final Report (Aptima): 3-5 days
Notifiable Result
See Link below for Notifiable Conditions for WA state labs:
https://doh.wa.gov/public-health-healthcare-providers/notifiable-conditions
See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:
https://www.doh.wa.gov/Portals/1/Documents/1200/LHJCommunicableDiseaseReporting.pdf