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HelpTest Code LAB3028 Extractable Nuclear Antigen Group (ENA)
Clinical System Name
Extractable Nuclear Antigen Group
Synonyms
Anti ENA Panel; Anti Sm; Anti Sm RNP; Anti SS-A (Anti-Ro); Anti SS-B (Anti-La); ENA Antibodies; SSA; SSB; ENA PANEL
Sample Requirements
Specimen: Whole Blood
Container(s): Gold SST, Red, Dark Green/Sodium Heparin, Lavender/EDTA
Preferred Vol: 3.0 mL
Minimum Vol: 1 mL
Note:
Processing Instructions
Reject due to:
Spin: Y
Aliquot: Y
Temp: 2-8 C
Storage location: CPA Fridge, FrigF Rack.
Off-site collection: Spin blood, refrigerate 1 mL serum/plasma in a polypropylene aliquot tube.
Stability
| Temperature | Time |
|---|---|
| Room temp | |
| Refrigerated | 7 d |
| Frozen | 6 m |
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M-F | 1-3 d |
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem East
Phone Number: (206) 987-2617
CPT Codes
86235 x5
Methodology
Method: Immunoassay on Bioplex 2200
Analytical Volume: 0.4 uL serum
Limitations:
Reference Range
| AI | |
|---|---|
| Negative | < 1.0 |
| Positive | > 1.0 |
Description
This assay is used in the detection and quantitation of autoantibodies to aid in the diagnosis of autoimmune disease. The ENA panel is intended for the detection of IgG class antibody to 5 separate analytes including SSA, SSB, Sm, RNP, and Sm/RNP in human sera. The results are semi-quantitative.