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HelpTest Code LAB3169 HIV 1/2 Ag Ab with Confirmation
Clinical System Name
HIV 1/2 Ag Ab with Confirmation
Synonyms
HIV Reflex
Sample Requirements
Specimen: Whole Blood
Container(s): Lavender/EDTA
Preferred Vol: 4 mL
Minimum Vol: 3 mL
Note: Other acceptable sample types include: Gold SST, Red, Gold Microtainer, Dark Green/Sodium Heparin, Lt. Green/Lithium Heparin Microtainer,
Specimens for this assay cannot not be shared with any other testing.
Processing Instructions
Reject due to:
Spin: Y
Aliquot:Y
Temp: 2 - 8 C
Storage location: Spin blood, refrigerate plasma in a plastic tube affixed with a large computer label in the CPA refrigerator. Store in CPA fridge, FrigF rack
1.5 mL plasma needed to complete all reflex testing
Off-site collection:
Stability
| Specimen Type | Temperature | Time |
|---|---|---|
| plasma | Room temp | 48 h |
| Refrigerated | 7 d | |
| Frozen | 2 m |
False positive results may occur if the plasma remains with the cells for >24 hours.
Availability
| STAT | Performed | TAT |
|---|---|---|
| N | M - F | 1-4 d |
Please contact the Chemistry Department (987-2565 or 987-3694) if STAT testing is requested. If Chemistry is unavailable (evenings), contact the clinical pathologist on call.
Performing Laboratory
Seattle Children's Laboratory
Department
Department: Chemistry
Location: Chem East
Phone Number: (206) 987-2617
CPT Codes
87389
Methodology
Method: Immunoassay on Bioplex 2200
Analytical Volume: 1.5 mL plasma needed to for all possible testing
Limitations:
Reference Range
|
Negative |
Description
This testing detects HIV-1 p24 Antigen in addition to HIV-1 and HIV-2 antibodies. The added detection of HIV-1 p24Ag shortens the antibody-negative window period associated with acute HIV-1 infection. Reactive samples will reflex to additional immunoassay testing for confirmation.
Reactive samples will be sent to UW for HIV 1 RNA testing.