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Test Code LAB3186 HSV Type 1 and 2 QuaNtitative PCR

Important Note

This testing is not available for CSF.  For CSF testing use the in-house HSV Qualitative PCR.

Buffy coat PCR is a special request done on known neonatal HSV infections.  For swabs, see "Herpes Group by PCR Qual, Tissue" to order and process.

Additional Codes

HSV12qN

Clinical System Name

HSV Type 1 and 2 QuaNtitative PCR

Synonyms

HSV PCR; HSV QUANTITATION; Herpes Simplex Virus; Herpes Simplex Virus by PCR; PCR for Herpes Simplex

 

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA, Red, or Gold/SST

Preferred Vol: 2.0 mL

Minimum Vol: 1.0 mL

 

Specimen: Eye Fluid

Container(s): Sterile Container

Preferred Vol: 0.5 mL

Minimum Vol: 0.5 mL

 

Note: This test cannot share with in-house Viral PCR. A separate tube needs to be collected if Hematology (CBC, Platelet, ESR, etc.) or in-house Microbiology testing is ordered.

Processing Instructions

Reject due to: Dark Green/Sodium Heparin

Spin: Y

Aliquot: Y

Temp: -20 C

Storage Location: Separate plasma or serum from cells ASAP or within two hours of collection. Transfer 1.0 mL (Min: 0.5 mL) plasma or serum to a plastic aliquot tube and freeze immediately at -20 C. Place sample in the CPA -20 freezer Send Outs rack.

 

Off-site Collection:

  • Blood: Separate plasma or serum from cells ASAP or within two hours of collection. Transfer 1.0 mL (Min: 0.5 mL) plasma or serum to a plastic aliquot tube and freeze immediately at -20 C.
  • CSF: Freeze within two hours of collection. If CSF has cells, spin and aliquot clear CSF to sterile container, freeze at -20 C.

Stability

Specimen Type Temperature Time
Blood or Body Fluid Room Temp Unacceptable
  Refrigerated < 48 h
  Frozen

Indefinite

Availability

STAT Performed TAT
N T, F 1 - 3 d

 

Note: Providers may request a STAT send out after hours. However, UW does not perform this test after hours. We will hold these samples until the normally scheduled batch times.

Performing Laboratory

University of Washington Medical Center

Department of Laboratory Medicine

Virology, Eastlake

1959 NE Pacific Street, Room NW120

Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

CPT Codes

87529 (x2)

Methodology

Method: Real-time Polymerase Chain Reaction

Analytical Volume: See Sample Requirements section.

Limitations:

Reference Range

None specified

 

Send Out Instructions

Reference Test Name:

HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid
Reference Lab Test Code:

HS12QN
Instructions:

Send out Monday through Sunday with the UW courier.