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Test Code LAB3186 HSV Type 1 and 2 QuaNtitative PCR

Important Note

This testing is not available for CSF.  For CSF testing use the in-house HSV Qualitative PCR.

Buffy coat PCR is a special request done on known neonatal HSV infections.  For swabs, see "Herpes Group by PCR Qual, Tissue" to order and process.

Additional Codes

HSV12qN

Clinical System Name

HSV Type 1 and 2 QuaNtitative PCR

Sample Requirements

Specimen: Whole Blood

Container(s): Lavender/EDTA, Red, or Gold/SST

Preferred Vol: 2.0 mL

Minimum Vol: 1.0 mL

 

Specimen: Eye Fluid

Container(s): Sterile Container

Preferred Vol: 0.5 mL

Minimum Vol: 0.5 mL

 

Note: This test cannot share with in-house Viral PCR. A separate tube needs to be collected if Hematology (CBC, Platelet, ESR, etc.) or in-house Microbiology testing is ordered.

Processing Instructions

Reject due to: Dark Green/Sodium Heparin

Spin: Y

Aliquot: Y

Temp: -20 C

Storage Location: Separate plasma or serum from cells ASAP or within two hours of collection. Transfer 1.0 mL (Min: 0.5 mL) plasma or serum to a plastic aliquot tube and freeze immediately at -20 C. Place sample in the CPA -20 freezer Send Outs rack.

 

Off-site Collection:

  • Blood: Separate plasma or serum from cells ASAP or within two hours of collection. Transfer 1.0 mL (Min: 0.5 mL) plasma or serum to a plastic aliquot tube and freeze immediately at -20 C.

Stability

Specimen Type Temperature Time
Blood or Body Fluid Room Temp Unacceptable
  Refrigerated < 48 h
  Frozen

Indefinite

Performing Laboratory

University of Washington Medical Center

Department of Laboratory Medicine

Virology, Eastlake

1959 NE Pacific Street, Room NW120

Seattle, WA 98195

 

Phone Number: (206) 520-4600

Department

Department: Send Outs

Phone Number: (206) 987-2563

Synonyms

HSV PCR; HSV QUANTITATION; Herpes Simplex Virus; Herpes Simplex Virus by PCR; PCR for Herpes Simplex

 

Availability

STAT Performed TAT
N T, F 1 - 3 d

 

Note: Providers may request a STAT send out after hours. However, UW does not perform this test after hours. We will hold these samples until the normally scheduled batch times.

Methodology

Method: Real-time Polymerase Chain Reaction

Analytical Volume: See Sample Requirements section.

Limitations:

Reference Range

None specified

 

CPT Codes

87529 (x2)

Send Out Instructions

Reference Test Name:

HSV Type 1 & 2 Quant PCR, Blood/Eye Fluid
Reference Lab Test Code:

HS12QN
Instructions:

Send out Monday through Sunday with the UW courier.