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Test Code LAB3723 VerifyNow P2Y12 Assay

Important Note

Call Core Coag lab (7-2561) and request a VerifyNow sample collection kit prior to draw. Do not share kit contents with the VerifyNow Aspirin Assay. Please request two kits. 

Do NOT use tube system to transport samples. Samples must be hand-carried to the laboratory. Do not agitate samples. Collect blood sample using largest bore needle possible. Butterfly needles (21 gauge) are ok to use.

Clinical System Name

VerifyNow P2Y12 Assay

Synonyms

Ticolpidine

VerifyNow PRU

Verify Now

Prasugrel

Ticlid

Clopidogrel

Plavix

Efient

Effient

Sample Requirements

Specimen: Whole blood

Container(s): 2 Greiner 3.2% Sodium Citrate Tubes.

Preferred Vol: (2) 2 mL tubes - please read instructions in collection kit before drawing.

Minimum Vol: N

 

 

Note: Greiner 3.2% sodium citrate tubes only. All other sodium citrate tube types are unaccpetable. Do NOT use tube system to transport samples. Samples must be hand-carried to the laboratory. Do not agitate the sample. Testing must be performed within 2 hours of collection. Sample cannot be used for any other testing

 

Line draws are not recommended for this test.

 

Preferred method of collection is venipuncture with vacuum fill. Test results are affected by incorrect blood volume. Use largest bore needle possible. Draw blood directly into vacutainer tubes, no syringe draws. Invert sample 4-5 times only.

Use of a Vascular Access Device for the collection of coag testing is not recommended. Please review the Coagulation Lab Collection Job Aid  and VerifyNow Collection Guide for more detailed instructions.  Blood must be transferred to the Greiner Citrate tubes by use of a blood transfer device to ensure proper fill. 

 

Lab:   Greiner Citrate tube kits are located in Core Coag Lab x72561

Processing Instructions

Reject due to:

  • Improper tubes.
  • Clotted, Hemolyzed, Insufficient quantity (under fill) or Overfill, and Improper collection.
  • Specimens tubes sent to the lab via the pneumatic tube transport system.
  • Specimen tubes that have been refrigerated, centrifuged, or greater than 2 hours old.

Spin: N

Aliquot: N

Storage location: Core 5 Fridge.

 

Note: Deliver blood to Core Coag bench. Coag Technologist will process.

 

Off-site collection: Cannot be collected outside Seattle Children's Main Campus. Do NOT collect at Seattle Children's Regional Clinic locations. Contact Laboratory for additional information.

Stability

Specimen Type Temperature Time
Whole Blood Room Temp ≤2 h
  Refrigerated N
  Frozen N

 

Availability

STAT Performed TAT
Y Daily 1 d

 

Performing Laboratory

Seattle Children's Laboratory

Department

Department: Coagulation

Phone Number: 206 987-2617 (Lab Client Services)

Methodology

Method: VerifyNow System

Analytical Volume: 2 tubes with 2.0 mL whole blood each

Limitations: Results could be affected by the following: GPIIb/IIIa inhibitors, low HCT, low PLT or inherited platelet disorders. Patients with HCT<20% or PLT<50 K/uL cannot be tested with the VerifyNow Assay. Results may be affected by improper specimen collection and transportation.

CPT Codes

85576

Reference Ranges

The expected range for patients not receiving P2Y12 inhibitors is 182-335 PRU. 

 

This test is not intended for therapeutic monitoring. 

Send Out Instructions

Reference Test Name: VerifyNow P2Y12 Assay
Reference Test Code: VERP2Y
Instructions: If received during business hours, notify Send Outs immediately. After hours or on weekends, fill out a UW General Lab Requisition, make a copy for Send Outs, and send sample(s) via Delivery Express DIRECT to HMC GWH 47.