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HelpTest Code LAB3748 M. tuberculosis complex DNA Detection
Infectious
Clinical System Name
M. tuberculosis complex DNA Detection
Synonyms
AFB PCR, broad range AFB PCR, broad range Mycobacteria PCR, hsp65, M. tuberculosis complex, Molecular AFB, Molecular Mycobacteria, Molecular Mycobacterium, MTB, MTB PCR, MTBC, Mycobacteria PCR, Mycobacterial identification, Mycobacterium identification, Mycobacterium tuberculosis complex identification, Mycobacterium tuberculosis complex PCR, TB PCR, TBC, Tuberculosis PCR, universal AFB PCR, universal Mycobacterium PCR
Sample Requirements
Specimen:
- Fluid (non-blood) from normally sterile sites (including Pleural Fluid)
- Gastrointestinal biopsies
- Gastric Aspirates (call Microbiology at ext. 7-2585 for Sodium Bicarbonate, see AFB Culture and Stain for collection instructions)
- Sterile Tissue (Fresh or paraffin embedded)
- Urine
Container: Sterile Screw-Capped Container
Preferred Vol: 1.0 mL of fluid; 1cc tissue preferred
Minimum Vol: Diagnostic yield generally proportional to specimen size.
Unacceptable: Tissues free-floating in formalin; samples containing SPS, citrate, or heparin; Blood/Serum specimens; samples collected on swabs (other than E-swabs)
See UW Test Catalog MTB complex PCR for the complete list.
Note: BAL should be sent as a respiratory specimen for Mycobact TB PCR Rapid Respiratory Xpert (LAB2504).
Processing Instructions
CPA: Forward all specimens to Microbiology for processing.
Microbiology: Keep frozen at -20C and handoff specimen to Send Outs (or CPA after hours) after completing requisition.
Send Outs: Send out Monday - Friday with the UW courier unless SCH Microbiology has indicated STAT testing. If STAT testing is indicated, send DIRECT to UW NW220 via Delivery Express courier. AFTER HOURS/WEEKENDS: Batch order to UW or send with manual requisition via DLX courier to NW220.
Stability
|
On-Site Collection |
|
|---|---|
| Temp | Time |
| Room temp | Unacceptable |
| Refrigerated | Unacceptable |
| Frozen | Indefinite |
Availability
Preliminary results in 2 to 4 days. Testing initiated upon receipt of specimen at UWMC Microbiology Molecular Diagnostics, Monday through Friday.
| STAT | Performed | TAT |
|---|---|---|
| N | M - F | 2 - 4 d |
Performing Laboratory
University of Washington Medical Center
Department of Laboratory Medicine
Clinical Microbiology Laboratory
1959 NE Pacific Street, Room NW120
Seattle, WA 98195
Phone Number:
- Microbiology Lab - (206) 598-6147
- UW Client Support Services (CSS) - (206) 520-4600
Department
Departments:
- Microbiology - (206) 987-2585
- Send Outs - (206) 987-2563
CPT Codes
87556
Send Out Instructions
| Reference Test Name: | M. tuberculosis complex DNA Detection |
| Reference Lab Test Code: | TBCDNA |
| Instructions: |
Send out Monday through Friday with the UW courier unless SCH Microbiology has indicated STAT testing. If STAT testing is indicated, send DIRECT to UW NW220 via Delivery Express courier.
Note: After hours and on weekends, all samples must be sent via Delivery Express to UW NW220. |
Description
This test is for molecular testing for R/O TB on direct patient material from non-respiratory sources. See Mycobact TB PCR Rapid Respiratory Xpert (LAB2504) for R/O TB on direct patient material from respiratory sources.
Notifiable Result
Mycobacterium tuberculosis is a notifiable condition under Washington State Law.
See Link below for Notifiable Conditions for WA State:
http://www.doh.wa.gov/PublicHealthandHealthcareProviders/NotifiableConditions.aspx
See Link below for PDF of phone numbers for Local Health jurisdictions (by County) in WA State:
https://www.doh.wa.gov/Portals/1/Documents/1200/LHJCommunicableDiseaseReporting.pdf