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Test Code TRH STIM TRH Stimulation - TSH

Clinical System Name

TRH Stimulation


thyrotropin releasing hormone

Sample Requirements

Specimen: Whole blood

Container(s): Gold SST, Red, Gold Microtainer, Dark Green/Sodium Heparin, Lt. Green/Lithium Heparin Microtainer

Preferred Vol: 1.0 mL

Minimum Vol: 0.5 mL


Note: Draw samples at baseline, 30, 60, 90, 120 minutes post TRH stimulation. Note collection times on samples and requisition.

Processing Instructions

Reject due to: gross lipemia

Spin: Y

Aliquot: Y

Temp: 2-8 C

Storage location: CPA refrigerator, FrigD rack. Serum/plasma should be removed from separator for storage.

Note: Minimum volume of 1.0 mL SERUM may be shared with TSH, T3, T4, free T3, and free T4.


Off-site collection: Spin blood. Transfer serum/plasma to plastic aliquot container. Refrigerate.


Specimen Type Temperature Time
gel separator with serum/plasma Refrigerated 48 h
separated serum/plasma Room temp 2 h
  Refrigerated 7 d
  Frozen -20 C 4 w



STAT Performed TAT
N M-F, day shift 1-4 d


Performing Laboratory

Seattle Children's Laboratory    


Department:  Chemistry

Location: Chem West

Phone Number:  (206) 987-2617




Reference Range

Refer to TSH for baseline reference values.


Method: Chemiluminescence by Vitros 3600, third generation assay

Analytical Volume: 0.200 mL serum/plasma

Limitations: Turbidity may affect results.

CPT Codes